gelatin hydrolysis test positive bacteria

(1966) Antibiotic susceptibility testing by a standardized single disk method. Clinical Laboratory Standards Institute CLSI document M2-A9, 1.īauer AW, Kirby WMN, Sherris JC, et al. Curr Protoc Microbio Chapter 6: Unit6A.5.Ĭlinical Laboratory Standards Institute (2006) Performance standards for antimicrobial disk susceptibility tests. Huq A, Haley BJ, Taviani E (2012) Detection, isolation, and identification of V. isolated from different types of water sources of Bangladesh and their characterization. (2019) Antibiotic resistance analysis of Vibrio spp. Sarkar MKD, Ahmmed T, Shahabuddin Md, et al. (2013) Prevalence of Vibrio cholera in different food samples in the city of Dhaka, Bangladesh. in fish and shellfish collected from the Swiss market. (2014) Contaminated pond water favors cholera outbreak at haibatpur village, purba medinipur district, west bengal, India. Gaffga NH, Tauxe RV, Mintz ED (2007) Cholera: a new homeland in Africa? Am J Trop Med Hyg 77: 705-713. Hardcopy of raw data used to deduct conclusion on this manuscript are currently available in our inventory and can be provided to the editors any given time when we are asked for it. Abdul Khalek, Mahmudul Hasan, Mohammad Shariful Islam had key role in data collection. Hasibul Hasan contributed in performing research, data collection and data input for this manuscript. Farhana Easmin and Nazmi Ara Rumi helps to write manuscript and correction also. Fozol Korim Ovi contributed in data analysis, statistics, and writing of the manuscript. Aoulad Hosen contributed as first author and was responsible for conception, experiment design, data collection and writing of the manuscript. The authors declare that there is no conflict of interest. All authors listed in this paper, was fully involved in the research and they contributed their full efforts, and revised the manuscript before final submission. doi: 10.3934/microbiol.2021028ĭepartment of Microbiology, Gono Bishwabidalay, Savar, Bangladesh, supported this research project.

in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile.

Abdul Khalek, Mahmudul Hasan, Farhana Easmin, Nazmi Ara Rumi, Mohammad Shariful Islam. Aoulad Hosen, Fozol Korim Ovi, Harunur Rashid, MD. Furthermore, we suggest selective use of sensitive antimicrobials listed here for therapeutics of cholera outbreak.Ĭitation: Md. Based on these data we recommend using tube-well water instead of river and pond water for drinking purposes. Isolates showed marginal sensitivity towards Tetracycline (33.33%), and Cephalexin (16.67%) and 100% resistance against antibiotics like Vancomycin, Penicillin, Erythromycin, and Nalidixic Acid. All the 12 isolates were sensitive to Gentamicin and ciprofloxacin (100%), while Chloramphenicol (91.67%), Sulfamethoxazole (91.67%), Azithromycin (66.67%) showed high sensitivity. than river and pond water (P 0.05) significantly in 5 different location the sample was collected from. Tube-well water has significantly lower concentration (log CFU/mL) of V. The results showed, out of 45 samples 12 contained V. cholera isolates to determine their antibiotic sensitivity profile. Following isolation antibiotic sensitivity test was performed on each V. cholera and was confirmed by analyzing their biochemical characteristics like Catalase, Oxidase, MR, VP, Indole, Sugar fermentation. Yellow colonies on TCBS agar were screed as V. cholera isolation water samples were first enriched in nutrient broth at 37 ☌ for 16 hours followed by cultivation in selective media TCBS agar at 37 ☌ for 24 hours. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of V. A total of 45 water samples were randomly collected for the isolation and identification of Vibrio spp. cholera in 3 different water sources River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. Therefore, the objective of this research was to isolate V. Serotype O1 of Vibrio cholera is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. Last cholera epidemic has been recorded in Bangladesh between 1992–1993, while few sporadic localized outbreaks have been reported as recent as 2005.

Gelatin hydrolysis test positive bacteria